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Table 6 Effect of interference on Δ R F , separation (α) and resolution (R S ) factors of the separated biosurfactants

From: Ionic liquid as separation enhancer in thin-layer chromatography of biosurfactants: mutual separation of sodium cholate, sodium deoxycholate and sodium taurocholate

Foreign substances

Ternary mixture

SDC-SC-STC

SDC-SC

SC-STC

Δ R F (0.52)

α (30.0)

R S (48.0)

Δ R F (0.36)

α (14.8)

R S (57.7)

Metal cations

 Cu2+

0.73

128.9

100

0.20

4.75

20.0

 Ni2+

0.66

71.8

94.4

0.21

4.09

23.3

 Th4+

0.73

148.4

102.4

0.18

4.71

20.0

Metal anions

 Br

0.73

148.4

97.3

0.14

2.75

16.4

 Cl

0.61

84.4

122.0

0.26

4.83

32.5

 CO3 2−

0.62

61.0

82.6

0.21

3.31

22.1

Vitamins

 Pyridoxine

0.47

47.1

72.3

0.32

4.52

35.5

 Thymine

0.43

40.1

61.4

0.31

3.93

34.4

 Ascorbic acid

0.43

21.4

53.7

0.33

4.48

36.6

Amino acids

 Lysine

0.66

73.4

69.4

0.25

4.88

25.0

 Glycine

0.69

122.5

115.0

0.19

3.63

22.3

 Valine

0.69

122.5

86.2

0.21

4.44

22.1

Non-ionic surfactants

 Brij 35

0.49

51.0

65.3

0.31

4.36

34.4

 Triton X 100

0.55

65.3

64.7

0.29

4.68

26.3

 Tween 20

0.53

60.4

75.7

0.35

7.36

31.1