Table 6 Robustness of the method by changing mobile phase composition ( n = 3) (concentration 100μg/mL)
From: Stability-indicating RP-HPLC method for the determination of 6-gingerol in polyherbal formulations
Mobile phase composition methanol:H 3 PO 4 in H 2 o (60:40 v / v ) | Mean area ± SD | Mean R t ± SD | %RSD of area | %RSD of R t | ||
|---|---|---|---|---|---|---|
Actual | Used | Level | ||||
60:40 | 58:42 | −2 | 9,867,378 ± 92,101 | 13.4 ± 0.26 | 0.93 | 1.9 |
60:40 | 0 | 9,870,953 ± 64,002 | 13.4 ± 0.1 | 0.65 | 0.75 | |
62:38 | +2 | 9,871,953 ± 108,701 | 13.6 ± 0.36 | 1.10 | 2.6 | |