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Journal of Analytical Science and Technology

Table 2 The Δ T m of protein at various concentrations of C8

From: Acquisition of data at multiple gains within a single thermal melt experiment using the Rotor-Gene Q instrument

[C8] mM

Δ T m at different levels of gain (°C)

Average Δ T m (°C)

−2

−1

0

1

1.3

1.7

2

2.3

3

3.5

4

5

0.25a

-

1.7

1.2

2.2

1.9

1.2

0.9

1

0.7

1.5

1

0.9

1.29 ± 0.45

0.75

1.7

2.7

2.9

2.7

2.7

2.7

1.7

2.2

1.7

1.5

1.7

2

2.22 ± 0.50

1

2.9

3

2.9

3

2.9

2.9

2.4

2.2

2.2

2.2

2.2

2.2

2.55 ± 0.36

1.5

2.7

3

3.5

3.2

2.7

2.9

2.7

2.7

2.7

2.7

2.5

2.5

2.82 ± 0.28

2

3.2

3.4

3.7

3.7

3.4

3.2

3.2

3.2

3

3

3.2

3

3.27 ± 0.23

2.5

3

3.4

3.7

3.5

3.4

3.2

3.2

3

2.9

3

2.9

2.9

3.19 ± 0.26

3

3.2

3.5

3.7

3.7

3.4

3.4

3.2

3.2

3.2

3.2

3

3

3.31 ± 0.23

3.5

3

3.7

3.7

3.7

3.5

3.4

3.4

3.2

3.2

3.2

3.2

3.2

3.40 ± 0.23

  1. a T m could not be calculated for −2 gain due to low fluorescence signal, and the melting curves were below threshold. ΔT m of protein for ten compounds (C1 to C10). The ΔT m of protein was measured at different gain levels set within the same experimental run that were used to determine the binding affinity; T m of the protein (T m ref) = 58.4 ± 0.2°C. The ΔT m were calculated by the Rotor-Gene Q-Pure Detection software version 2.0.2 (Build 4).
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