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Table 2 The Δ T m of protein at various concentrations of C8

From: Acquisition of data at multiple gains within a single thermal melt experiment using the Rotor-Gene Q instrument

[C8] mM Δ T m at different levels of gain (°C) Average Δ T m (°C)
−2 −1 0 1 1.3 1.7 2 2.3 3 3.5 4 5
0.25a - 1.7 1.2 2.2 1.9 1.2 0.9 1 0.7 1.5 1 0.9 1.29 ± 0.45
0.75 1.7 2.7 2.9 2.7 2.7 2.7 1.7 2.2 1.7 1.5 1.7 2 2.22 ± 0.50
1 2.9 3 2.9 3 2.9 2.9 2.4 2.2 2.2 2.2 2.2 2.2 2.55 ± 0.36
1.5 2.7 3 3.5 3.2 2.7 2.9 2.7 2.7 2.7 2.7 2.5 2.5 2.82 ± 0.28
2 3.2 3.4 3.7 3.7 3.4 3.2 3.2 3.2 3 3 3.2 3 3.27 ± 0.23
2.5 3 3.4 3.7 3.5 3.4 3.2 3.2 3 2.9 3 2.9 2.9 3.19 ± 0.26
3 3.2 3.5 3.7 3.7 3.4 3.4 3.2 3.2 3.2 3.2 3 3 3.31 ± 0.23
3.5 3 3.7 3.7 3.7 3.5 3.4 3.4 3.2 3.2 3.2 3.2 3.2 3.40 ± 0.23
  1. a T m could not be calculated for −2 gain due to low fluorescence signal, and the melting curves were below threshold. ΔT m of protein for ten compounds (C1 to C10). The ΔT m of protein was measured at different gain levels set within the same experimental run that were used to determine the binding affinity; T m of the protein (T m ref) = 58.4 ± 0.2°C. The ΔT m were calculated by the Rotor-Gene Q-Pure Detection software version 2.0.2 (Build 4).