Plot of Δ
vs concentration of ten compounds C1 to C10. Ten compounds C1 to C10 were tested at three different concentrations of 0.5, 2, and 5 mM. The higher shift in ΔT
m for higher concentration of compound signifies binding. T
m of the protein (T
ref) = 58.4 ± 0.2°C. The compound C7 was insoluble at 2- and 5-mM concentrations.