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Figure 3 | Journal of Analytical Science and Technology

Figure 3

From: Acquisition of data at multiple gains within a single thermal melt experiment using the Rotor-Gene Q instrument

Figure 3

Plot of Δ T m vs concentration of ten compounds C1 to C10. Ten compounds C1 to C10 were tested at three different concentrations of 0.5, 2, and 5 mM. The higher shift in ΔT m for higher concentration of compound signifies binding. T m of the protein (T m ref) = 58.4 ± 0.2°C. The compound C7 was insoluble at 2- and 5-mM concentrations.

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