Figure 2
Fluorescence melting curves at gains 2 and 6. (A) The fluorescence melting curves for the ten compounds C1 to C10 at gain 2 are shown. It was optimal for most samples that had relatively higher fluorescence yield. (B) Melting curves at gain 6 show optimal melting transition for samples with low fluorescence yield while samples with high fluorescence yield showed saturation of fluorescence intensity, and hence, their ΔTm could not be calculated at this gain setting.