Figure 5

Capillary electrophoresis chromatogram of the osmolytes in the leaves of Limonium suffruticosum grown in saline soil. Plant leaf samples were esterified with 4-bromophenacyl bromide as described in the ‘Methods’ section. The samples were injected hydrostatically (25 mm, 60 s) at 25°C. The applied potential was 20 kV, and the peaks were monitored at 262 nm. The chromatogram showed the accumulated osmolytes in the plant samples alongside with authentic standard peaks of trimethylamine (TMA) and β-alanine betaine (β-AB).