Table 1 Optimized chromatographic conditions
Stationary phase (column) | Phenomenex luna C1 (250 × 4.5 mm) packed with 5 μmparticles |
|---|---|
Mobile phase | Acetonitrile, 0.2 M phosphate buffer (pH 7.4) 40:60 (v/v) |
Detection wave length (nm) | 228 |
Run time (min) | 10 |
Flow rate (mL/min) | 1 |
Volume of injection loop (μL) | 20 |
Column temperature | Ambient |
Glimepiride R t (min) | 3.543 |