Method development for simultaneous detection of ferulic acid and vanillin using high-performance thin layer chromatography

Journal of Analytical Science and Technology

Table 4 Robustness of FA & Vanillin

Parameters	FA		Vanillin
Parameters	SD^a	%RSD^b	SD^a	%RSD^b
Mobile phase (toluene/1,4-dioxan/acetic acid) (9{±1}:2.5{±1}:0.4{± 0.2}) (v/v)	2.95 ± 0.13	0.039	2.526 ± 0.05	0.039
Mobile phase vols. 10, 12 and 14 ml	3.00 ± 0.09	0.027	3.626 ± 0.12	0.056
Saturation time 10, 15 and 20 min	2.20 ± 0.06	0.027	2.699 ± 0.07	0.058
Temperature 25, 30 and 37°C	2.25 ± 0.12	0.050	3.351 ± 0.10	0.052

^aSD, standard deviation of peak area (n = 3); ^bRSD, percent relative standard deviation (n = 3).