NMR studies of a Glutaredoxin 2 from Clostridium oremlandii

Lee, Eun Hye; Cheong, Hae-Kap; Kim, Hye-Yeon

doi:10.1186/2093-3371-4-2

Journal of Analytical Science and Technology

Table 1 Assigned backbone chemical shifts (1HN, 15 N, 13CO, 13Cα and 13Cβ) of the C15S mutant

From: NMR studies of a Glutaredoxin 2 from Clostridium oremlandii

AA	HN	N	Cα	Cβ	CO	AA	HN	N	Cα	Cβ	CO
K2	-	-	53.11	31.01	172.9	A46	7.573	119.8	51.64	15.38	176.3
N3	9.051	121.6	50.24	36.27	171.9	K47	7.341	116.7	55.65	30.68	175.9
I4	8.905	129.5	58.24	36.53	172.6	T48	8.153	107.8	59.14	68.14	173.8
T5	9.033	124.7	58.69	68.94	169.6	G49	8.49	110.5	42.89	-	171.7
I6	9.087	123.6	55.28	39.13	169.5	W50	8.535	122.5	53.42	27.5	172.8
Y7	8.926	129.2	54.69	36.8	173.5	D51	8.356	118.9	49.69	38.2	173.4
T8	8.784	111.8	57.11	69.57	170.6	T52	7.153	108	57.08	68.83	170.9
K9	6.958	112.2	52.91	34.43	175.1	V53	8.021	110.5	55.83	30.89	170
N10	8.323	119.2	52.42	35.54	172.7	P54	-	-	59.2	33.52	173.8
P13	-	-	61.69	29.38	176.9	Q55	7.844	116.5	54.31	31	172
Y14	8.659	126.1	58.27	35.19	176.2	V56	8.518	123.9	59.31	30.42	170.6
S15	11.18	130.2	61.41	51.86	172.3	F57	9.482	126.2	53.6	40.89	172.5
K16	7.612	120.4	56.83	29.69	176.5	V58	8.779	118.8	58.27	30.67	173.3
K17	7.694	120.4	56.84	30.24	176.4	D59	9.754	130	54.44	36.77	173.1
A18	8.277	122.1	52.92	16.91	175.6	E60	-	-	54.66	26.15	172.9
V19	8.354	118.1	64.72	28.92	175.6	E61	8.533	123.4	53.16	28.25	172.4
S20	8.237	116.1	59.22	59.93	174.3	F62	8.81	127.5	52.56	36.32	172.9
L21	7.68	123.4	55.33	37.92	177.2	L63	8.59	128.3	51.63	40.3	172.3
L22	7.636	118.8	55.57	37.86	176.6	G64	5.136	102.3	41.28	41.28	169.3
S23	8.838	114.5	59.36	60.24	175.3	G65	8.823	108.3	40.98	41.03	171.3
S24	8.109	118.4	58.38	60.28	172.7	C66	8.721	119.9	60.96	35.07	-
K25	7.329	119.3	52.69	30.37	174.8	D67	-	-	55.04	36.66	176.3
G26	7.779	106	43.67	-	171.7	D68	7.602	118.7	54.92	38.57	176.4
V27	7.012	112	56.67	30.7	172.2	I69	8.009	111.5	62.98	35.16	175.1
D28	8.438	123.8	51.44	38.58	173.1	H70	7.594	120.9	59.86	25.44	175
F29	7.711	118	52.92	39.09	169.9	A71	8.196	124.9	52.91	14.83	178
K30	8.796	122.4	51.96	31.66	171.5	L72	8.091	116.4	54.66	39.86	177.1
E31	8.648	127.8	51.43	28.48	173	D73	7.863	121	54.4	39.05	177
V32	9.056	130.8	58.79	29.63	171.2	R74	8.153	120.6	56.52	27.33	175.8
D33	8.44	126.3	50.56	38.56	176	Q75	7.462	114.5	53.4	27.95	173.6
V34	9.159	120.7	58.59	28.05	173.5	G76	7.854	107.9	42.97	-	172.2
T35	8.458	119.7	64.95	66.01	172.4	I77	7.713	115.7	58.9	36.87	174
H36	8.335	116.7	52.67	28.19	172.2	L78	7.18	123.4	55.31	37.16	175.1
D37	7.393	120.4	49.88	37.87	172.1	D79	8.68	118.1	55.5	37.08	175.5
S38	8.297	118.8	58.21	60.13	174.5	K80	7.165	117.6	56.33	29.42	177.8
K39	8.33	124.3	56.29	28.85	176	K81	7.843	119.8	55.12	29.3	175.3
A40	7.775	120.8	51.76	15.74	178.1	L82	7.896	113.1	52.22	39.01	173.2
F41	7.683	116.6	56.17	36.15	174.1	G83	7.521	104.5	42.96	-	171.8
E42	8.404	120.2	57.2	26.4	176.8	L84	7.835	119.5	52.36	39.76	173.9
D43	7.692	118.8	54.38	37.61	175.6	K85	8.096	120.6	52.74	30.37	173
V44	7.135	121	63.45	28.52	174.6	L86	8.2	123.9	52.29	39.69	174.5
M45	8.028	119.4	56.38	30.65	177	E87	8.413	121.4	53.69	27.68	173.3

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