Parameter | Study condition | Mean area???SD | %RSD of area | R t???SD | % RSD of R t | ||
---|---|---|---|---|---|---|---|
Original | Used | Level | |||||
Mobile phase ratio (ACN/water) | 50:50 | 48:52 | ?1 | 2218634.9???6686.7 | 0.30 | 7.968???0.024 | 0.30 |
50:50 | 0 | ||||||
52:48 | +1 | ||||||
Flow rate (mL?min?1) | 1.0 | 0.9 | ?1 | 2216384.3???8147.4 | 0.37 | 7.958???0.068 | 0.85 |
1.0 | 0 | ||||||
1.1 | +1 | ||||||
Detection wavelength (nm) | 280 | 278 | ?1 | 2214941.3???13356.9 | 0.60 | 7.967???0.030 | 0.38 |
280 | 0 | ||||||
282 | +1 |