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Table 5 Robustness data of the HPLC method

From: Development and validation of RP-HPLC-PDA method for the quantification of eugenol in developed nanoemulsion gel and nanoparticles

Parameter

Study condition

Mean area???SD

%RSD of area

R t???SD

% RSD of R t

Original

Used

Level

Mobile phase ratio (ACN/water)

50:50

48:52

?1

2218634.9???6686.7

0.30

7.968???0.024

0.30

50:50

0

52:48

+1

Flow rate (mL?min?1)

1.0

0.9

?1

2216384.3???8147.4

0.37

7.958???0.068

0.85

1.0

0

1.1

+1

Detection wavelength (nm)

280

278

?1

2214941.3???13356.9

0.60

7.967???0.030

0.38

280

0

282

+1

  1. n?=?3 at each level. Concentration?=?100??g?mL?1.