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Table 1 Mobility of amino acids in terms of R F values using different mobile phases

From: Environmentally preferable solvents promoted resolution of multi-component mixtures of amino acids: an approach to perform green chromatography

Amino acids Mobile phases (M)
M 1 M 2 M 3 M 4 M 5 M 6 M 7 M 8 M 9 M 10 M 11 M 12
Leucine 0.98 0.14 0.01 0.91 0.71 0.92 0.77 0.82 0.82 0.75 0.87 0.87
Isoleucine 0,97 0.21 0.01 0.92 0.72 0.92 0.76 0.81 0.81 0.77 0.85 0.81
Phenyl alanine 0.99 0.26 0.01 0.90 0.74 0.90 0.78 0.81 0.88 0.78 0.88 0.89
Tyrosine 0.97 0.22 0.02 0.92 0.75 0.91 0.80 0.83 0.86 0.77 0.90 0.88
Alanine 0.93 0.14 0.01 0.90 0.54 0.91 0.66 0.67 0.70 0.70 0.77 0.68
Lysine 0.97 0.06 0.01 0.27 0.07 0.31 0.60 0.10 0.08 0.11 0.08 0.07
Proline 0.92 0.10 0.01 0.75 0.47 0.76 0.36 0.45 0.46 0.53 0.58 0.58
Serine 0.94 0.14 0.01 0.88 0.61 0.91 0.59 0.65 0.70 0.71 0.75 0.68
Glutamic acid 0.95 0.17 0.01 0.70 0.68 0.77 0.54 0.47 0.41 0.58 0.64 0.49
Methionine 0.92 0.10 0.01 0.84 0.68 0.89 0.69 0.72 0.75 0.66 0.85 0.81
Arginine 0.92 0.07 0.01 0.24 0.05 0.28 0.09 0.10 0.06 0.05 0.07 0.05
Histidine 0.92 0.11 0.01 0.79 0.40 0.85 0.55 0.64 0.48 0.16 0.66 0.54
Tryptophan 0.99 0.14 0.01 0.83 0.77 0.89 0.63 0.83 0.88 0.78 0.88 0.83