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Journal of Analytical Science and Technology
Fig. 2 | Journal of Analytical Science and Technology

Fig. 2

From: Sensitive and reliable miRNA analysis based on cyclic reverse transcription and CRISPR-Cas12a-assisted signal cycle

Fig. 2

Feasibility of the CRT and the trans-cleavage activity of Cas12a/crRNA. A fluorescence spectrum of the FAM labeled-miRNA before and after hybridizing with the primer sequences. B Fluorescence intensities of the FAM labeled-miRNA during the CRT process when the reverse transcriptase existed. C Fluorescence intensity of the H probe during the trans-cleavage activity of the Cas12a/crRNA. 1, H probe (linear); 2, H probe (hairpin structure); 3, H probe (hairpin structure) + Cas12a/crRNA; 4, H probe (hairpin structure) + Cas12a/crRNA + CRT product

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