Fig. 3
From: Method development for 14C-labeling of IgG antibodies in preparation for clinical trials
Protein concentration, radioactive concentration, specific activity, 14C-labeing efficiencies, and antibody activity test. a Protein concentration determined by Bradford assay, radioactive concentration measured by LSC, specific activities and 14C-labeing efficiencies calculated by following two formulas (1 and 2). b Plot between labeling efficiency and 14C-formamide molar equiv. c Antibody activities detected by the dot blot method. Mouse IgG was used as an antigen and concentration dependent antigens were detected by 14C-labeled anti-mouse rabbit IgG with different labeling efficiencies. Alkaline Phosphatase conjugated anti-rabbit goat IgG was used as the second antibody. Each antibody concentration was estimated by the intensity of dot blot. d The dot intensities were analyzed by ImageJ software (National Institute of Health (NIH), Bethesda, USA), visually represented as indicated, and then compared with the antibody activity in each reaction condition