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Journal of Analytical Science and Technology
Fig. 3 | Journal of Analytical Science and Technology

Fig. 3

From: Novel plasma-polymerized coating facilitates HeLa cell spheroid formation, exerting necroptosis via β-cyclodextrin-encapsulated resveratrol

Fig. 3

Development and size distribution analysis of HeLa cell spheroids on ppOMBTSTS surface. A Three thousand HeLa cells were added to Matrigel (provided by BME) or a PECVD-coated plate and further incubated for 5 days. Spheroids developed in two different conditions were observed under a microscope. Representative images are displayed in the top panel and the spheroid count was plotted as bar graphs (mean ± SE, n = 3). p-values were calculated using Student’s t-test. B HeLa cell spheroids were grown on PECVD-coated plates for 10 days. Spheroids were observed daily under a microscope, and random images were captured. Subsequently, the count and diameters of the spheroids were measured. Representative images are presented in the top panel. Spheroid diameters were plotted against incubation days (mean ± SE, n = 8–15). C Size distributions of HeLa cell spheroids, developed as described in panel B, were replotted against incubation periods

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