Fig. 2

Feasibility of the proximity-CHA and DNAzyme-assisted signal amplification. A Fluorescent spectrum of the FAM labeled H1 probe and Cy5 labeled H2 probe before and after assembly. 1: H1 probe (linear), 2: H1 probe (hairpin), 3: H2 probe (linear), 4: H2 probe (hairpin). B Fluorescent spectrum of the FAM labeled H1 probe and Cy5 labeled H2 probe before and after assembly. A: FAM-H1, B: FAM-H1 + template sequence, C: Cy5-H2, D: Cy5-H2 + template sequence. C Fluorescent spectrum of the FAM labeled H3 probe with the “a” fragment similar or different with the target miRNA