Table 3 Inter- and Intra-day accuracy and precision, recovery (RE), matrix effect (ME), process efficiency (PE), and autosampler stability (AS) of target compounds in hair
Analyte | QC sample (pg/mg) | Inter-day (n = 24) | Intra-day (n = 6) | RE (n = 6) (%) | ME (n = 6) (%) | PE (n = 6) (%) | AS stability (n = 6) (%) | ||
|---|---|---|---|---|---|---|---|---|---|
% bias | % CV | % bias | % CV | ||||||
EtG | 7 | − 9.3 | 14.4 | 2.3 | 10.3 | – | – | – | – |
20 | 0.7 | 4.0 | 4.5 | 4.8 | 89.2 | 91.4 | 81.5 | 109.7 | |
100 | − 1.8 | 1.4 | − 2.7 | 3.7 | 98.5 | 88.0 | 86.7 | – | |
500 | − 3.9 | 1.0 | − 5.4 | 5.9 | 104.5 | 96.6 | 100.9 | 101.2 | |
COC | 2 | − 3.1 | 3.9 | 1.9 | 5.9 | – | – | – | – |
5 | 3.0 | 0.6 | 4.0 | 1.4 | 100.5 | 81.7 | 82.0 | 96.6 | |
25 | 3.5 | 1.4 | 2.0 | 0.5 | 100.0 | 81.6 | 81.6 | – | |
125 | − 4.1 | 1.5 | − 6.1 | 1.6 | 99.6 | 83.1 | 82.8 | 100.8 | |
CE | 10 | 3.4 | 4.3 | 9.7 | 10.0 | – | – | – | – |
25 | 3.6 | 2.1 | 5.7 | 7.2 | 104.8 | 96.2 | 100.8 | 99.9 | |
125 | 3.6 | 2.2 | 2.7 | 1.8 | 97.2 | 89.9 | 87.3 | – | |
625 | − 1.5 | 2.6 | − 4.1 | 0.9 | 100.1 | 82.9 | 83.0 | 100.3 | |
BZE | 1 | − 4.4 | 7.7 | 6.4 | 8.4 | – | – | – | – |
2.5 | 3.7 | 3.2 | 8.5 | 1.9 | 97.5 | 105.1 | 102.5 | 99.6 | |
12.5 | 8.3 | 4.8 | 5.1 | 3.9 | 96.6 | 101.9 | 98.4 | – | |
62.5 | − 0.1 | 3.3 | − 3.4 | 2.8 | 101.7 | 105.4 | 107.1 | 99.9 | |