Fig. 4

Plk1 delocalized from the kinetochore of HeLa cells. The cells were treated with DMSO, nocodazole (200 nM), BI2536 (200 nM), and their derivatives (200 μM) for 24 h a Representative images show kinetochore (green) and Plk1 (red) fluorescence among prometaphase cells. The phase of the cell was determined by the shape of the DNA (blue). b The quantitation graph of Plk1 fluorescence intensity at the kinetochore. The intensity of Plk1 was measured 10 times per cell. “n” represents the cell number