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Journal of Analytical Science and Technology
Fig. 2 | Journal of Analytical Science and Technology

Fig. 2

From: Simple, sensitive, and label-free miRNA analysis through strand displacement reaction integrating with G-quadruplex-based signal generation

Fig. 2

Feasibility study of the established approach. A Schematic illustration of the fluorescence assay to test the feasibility. B Fluorescence spectrum of the Cy3 labeled hairpin probe (H probe, Hp) during the construction of the detection probe. curve 1: Cy3 labeled H probe (linear), curve 2: Cy3 labeled H probe (assembled to hairpin structure), curve 3: Cy3 labeled H probe (assembled to hairpin structure) + BHQ-labeled “5” sequence, curve 4: Cy3 labeled H probe (linear) + BHQ-labeled “5” sequence. C Fluorescence spectrum of the Cy3 labeled H probe during the target recycling process. Curve a: detection scaffold, curve b: detection scaffold + miRNA, curve c: detection scaffold + miRNA + catalytic chain, curve d: detection scaffold + miRNA + catalytic chain + DNA polymerase. D ThT signals of the approach when miRNA-21 and ThT existed or not

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