Table 4 Accuracy and precision of LQC and HQC samples to study the matrix effect of the seven bile acids and their respective internal standards (n = 6)
Analytes | Mean value | IS (100 ng/mL) | Mean value | |||
|---|---|---|---|---|---|---|
LQC | HQC | |||||
CA | Accuracy (%bias) | 9.43 | 12.66 | d4-CA | Accuracy (%bias) | − 10.34 |
Precision (%CV) | 4.10 | 3.05 | Precision (%CV) | 6.74 | ||
CDCA | Accuracy (%bias) | 9.41 | 8.60 | d4-DCA | Accuracy (%bias) Precision (%CV) | − 13.55 11.81 |
Precision (%CV) | 9.88 | 4.87 | ||||
DCA | Accuracy (%bias) | 12.77 | − 12.85 | |||
Precision (%CV) | 10.14 | 4.31 | ||||
GDCA | Accuracy (%bias) | 13.05 | 12.09 | d4-GDCA | Accuracy (%bias) | − 4.42 |
Precision (%CV) | 10.59 | 3.08 | Precision (%CV) | 7.51 | ||
TDCA | Accuracy (%bias) | 12.37 | 13.86 | d4-TDCA | Accuracy (%bias) | − 8.18 |
Precision (%CV) | 7.74 | 3.61 | Precision (%CV) | 9.36 | ||
Tα-MCA | Accuracy (%bias) | 7.60 | 10.76 | d4-TCA | Accuracy (%bias) Precision (%CV) | − 11.36 11.70 |
Precision (%CV) | 6.80 | 7.42 | ||||
Tβ-MCA | Accuracy (%bias) | 6.25 | 12.65 | |||
Precision (%CV) | 11.91 | 3.97 | ||||