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Journal of Analytical Science and Technology

Table 3 Extraction recoveries of the seven bile acids and their respective internal standards in the developed method (n = 6)

From: Simultaneous determination of seven bile acids to study the effect of ivermectin on their plasma levels in rat by UHPLC–MS/MS

Analytes

%Recovery

Analyte at QC levels

IS used

IS (100 ng/mL)

IS normalized

LQC

MQC

HQC

LQC

MQC

HQC

CA

Mean (%)

81.72

76.40

79.37

d4-CA

91.78

89.03

93.24

86.47

%CV

12.08

3.74

11.47

2.50

12.08

3.74

11.47

CDCA

Mean (%)

87.22

79.84

80.45

d4-DCA

81.22

3.21

107.38

98.30

99.05

 

%CV

13.25

5.16

10.36

13.25

5.16

10.36

DCA

Mean (%)

74.14

80.60

86.98

91.28

99.23

107.09

 

%CV

6.38

6.37

12.17

6.38

6.37

12.17

GDCA

Mean (%)

76.61

75.29

78.78

d4-GDCA

88.33

86.73

85.23

89.18

 

%CV

11.64

3.74

11.58

2.06

11.64

3.74

11.58

TDCA

Mean (%)

76.61

84.74

87.09

d4-TDCA

86.14

88.93

98.37

101.10

 

%CV

10.49

3.28

9.92

3.44

10.49

3.28

9.92

Tα-MCA

Mean (%)

79.08

80.06

87.86

d4-TCA

92.98

4.72

85.05

86.10

94.49

%CV

10.05

11.74

11.22

10.05

11.74

11.22

Tβ-MCA

Mean (%)

76.61

83.49

87.71

82.39

89.79

94.51

%CV

11.76

4.87

11.45

11.76

4.87

11.45

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