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Journal of Analytical Science and Technology
Fig. 1 | Journal of Analytical Science and Technology

Fig. 1

From: Effective investigation of murine femoral bone development utilizing correlative light and electron microscopy (CLEM)

Fig. 1

Preparation of femoral bone for CLEM investigation and utilization of the CLEM holder for various instruments. In order to maintain the correlative aspects of CLEM, observation sections from the regions of interest (ROIs) of LM specimens, bone blocks, and UTS specimens were carefully prepared and were analyzed via each instrument from LM to EM. The CLEM holder is capable of loading nine UTS specimens and is applicable to a variety of SEM instruments with STEM and EDS detectors installed. The holder loaded with TEM specimens is easily transferable for LM which is analyzed using either transmitted or reflected light, which enhances the efficacy of CLEM. (EM: Electron microscopy. LM: Light microscopy. UTS: Ultra-thin sections)

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