Liquid chromatography-tandem mass spectrometry for pharmacokinetics evaluation of AZD5305, a selective PARP1 inhibitor, in mice

Journal of Analytical Science and Technology

Table 2 Stability of AZD5305 in mice plasma (mean ± SD, n = 5)

Storage conditions	Nominal concentration (ng/mL)	Stability (%)
Processed sample (autosampler, 10 °C, 24 h)	3	95.1 ± 5.22
Processed sample (autosampler, 10 °C, 24 h)	900	98.9 ± 5.71
Long-term (2 weeks at − 20 °C)	3	96.9 ± 4.73
Long-term (2 weeks at − 20 °C)	900	96.8 ± 1.82
Long-term (4 weeks at − 20 °C)	3	97.5 ± 2.54
Long-term (4 weeks at − 20 °C)	900	98.9 ± 3.92
Freeze–thaw (3 cycles, − 20 °C → RT)	3	96.6 ± 9.10
Freeze–thaw (3 cycles, − 20 °C → RT)	900	99.7 ± 2.69
6 h after room temperature (25 °C)	3	99.1 ± 4.37
6 h after room temperature (25 °C)	900	98.5 ± 1.40