Table 1 Intra- and inter-day accuracy and precision of the LC–MS/MS method
Nominal concentration (ng/mL) | Measured concentration (ng/mL) | Accuracy (%RE) | Precision (%CV) |
|---|---|---|---|
Mouse plasma | |||
Intra-day (n = 5) | |||
1 | 1.01 ± 0.0615 | 0.917 | 6.09 |
3 | 2.91 ± 0.178 | 3.06 | 6.12 |
30 | 27.3 ± 0.917 | 8.94 | 3.36 |
900 | 828 ± 59.9 | 8.00 | 7.24 |
Inter-day (n = 15) | |||
1 | 1.00 ± 0.101 | 0.172 | 10.1 |
3 | 2.90 ± 0.240 | 3.24 | 8.26 |
30 | 29.2 ± 2.03 | 2.78 | 6.94 |
900 | 904 ± 85.6 | 0.444 | 9.47 |