Fig. 5

Membrane penetration of SYTOX green and time-dependent peptide translocation. A SYTOX green uptake of S. aureus caused by the peptides. Fluorescence was measured at the indicated times. B Ability of peptides to translocate into liposomes. A decrease in fluorescence intensity after addition of a peptide is indicative of digestion of the internalized peptide. Membrane translocation of the peptides was measured by resonance energy transfer from Trp to DNS-PE. The lipid and peptide concentrations were 100 and 1 µM, respectively