Trail no. | Alcoholic modifiers | Alcoholic modifier (×) per 100 mL of mobile phase (mL) | Observations |
---|---|---|---|
1 | Methanol | 15 | Poor resolution between the enantiomers, tailing of peaks observed |
2 | Ethanol | 15 | Enantiomers eluted very lately and the peak shape is broad. |
3 | Isopropanol | 15 | Enantiomers eluted early but not separated completely; no resolution between the peaks. |
4 | n-Butanol | 15 | No proper separation of enantiomers |
5 | Ethanol:isoproponol | 7.5:.5 | Enantiomers eluted early with good resolution and capacity factor. |