Table 1 Multiple reaction monitoring (MRM) transitions with optimized fragmentation voltage and collision energy
Compound | RT (min) | MW | ISTD | MRM transition ( m / z ) | Fragmentor (V) | CE (eV) |
|---|---|---|---|---|---|---|
Opiates | ||||||
6-Acetylmorphine | 1.73 | 327.2 | Oxycodone-d3 | 328.2 > 165.1 | 173 | 48 |
328.2 > 211.1 | 173 | 24 | ||||
Buprenorphine | 3.27 | 467.3 | Buprenorphine-d4 | 468.3 > 55.1 | 208 | 44 |
468.3 > 396.2 | 208 | 40 | ||||
Hydromorphone | 0.92 | 285.2 | Hydromorphone-d3 | 286.2 > 185.1 | 158 | 28 |
286.2 > 128.1 | 158 | 64 | ||||
Norbuprenorphine | 2.70 | 413.3 | Norbuprenorphine-d3 | 414.3 > 152.1 | 235 | 100 |
414.3 > 165.1 | 235 | 104 | ||||
Oxycodone | 1.69 | 315.2 | Oxycodone-d3 | 316.2 > 241.1 | 178 | 28 |
316.2 > 256.1 | 178 | 24 | ||||
Oxymorphone | 0.75 | 301.1 | Oxymorphone-d3 | 302.1 > 284.2 | 179 | 16 |
302.1 > 227.1 | 179 | 28 | ||||
Morphine | 0.7 | 285.2 | Morphine-d3 | 286.2 > 165.1 | 161 | 48 |
286.2 > 157.1 | 161 | 44 | ||||
Hydrocodone | 1.74 | 299.2 | Hydrocodone-d3 | 300.2 > 199.1 | 167 | 28 |
300.2 > 171.1 | 167 | 40 | ||||
Benzodiazepines | ||||||
Alpha hydroxy alprazolam | 3.35 | 324.1 | Alpha hydroxy alprazolam-d5 | 325.1 > 216.1 | 105 | 40 |
325.1 > 279.1 | 105 | 40 | ||||
Alprazolam | 3.69 | 308.1 | Alprazolam-d5 | 309.1 > 281.1 | 99 | 28 |
309.1 > 205.1 | 99 | 44 | ||||
Nordiazepam | 3.72 | 270.1 | Temazepam-d5 | 271.1 > 140.1 | 161 | 24 |
271.1 > 208.1 | 161 | 28 | ||||
Oxazepam | 3.38 | 286.1 | Oxazepam-d5 | 287.1 > 241.1 | 167 | 16 |
287.1 > 269.1 | 167 | 8 | ||||
Temazepam | 3.73 | 300.1 | Temazepam-d5 | 301.1 > 255.1 | 198 | 20 |
301.1 > 177.1 | 198 | 40 |